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Location

CoLab

Start Date

3-5-2019 10:30 AM

End Date

3-5-2019 11:45 AM

Document Type

Poster

Description

On February 15, 2019 I obtained a soil sample about 6 inches below the surface from my side yard in Lenexa, KS at 38⁰ 58’ 26’’ N, 94⁰ 43’ 54’’ W in freezing rain. The soil consisted of a mixture of clay and planting soil. I took the sample to be tested for antibiotic producing colonies against the 6 safe relatives to ESKAPE pathogens. The soil was found to have a pH of 5, and then using aseptic technique, serial dilutions were preformed and transferred to 50% TSA agar plates for growth. Plates at a 10⁻2 dilution grew the most statistically numerical colonies. A total of 12 isolated colonies were selected and grown on master plates. Each colony was then tested against the 6 safe relatives to ESKAPE pathogens on antibiotic screening plates. Two colonies produced zones of inhibition against Enterococcus faecalis, one of which was selected as the strongest candidate and a quadrant streak was performed. From the quadrant streak an isolated colony in quadrant 3 was used for Gram staining revealing a Gram-negative short bacillus. To learn more about the DNA of the bacteria, Polymerase Chain Reaction (PCR) and gel electrophoresis analysis were performed. The 16s rRNA gene will be sequenced to determine the bacteria’s genus.

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May 3rd, 10:30 AM May 3rd, 11:45 AM

Search for Antibiotics in Soil Samples

CoLab

On February 15, 2019 I obtained a soil sample about 6 inches below the surface from my side yard in Lenexa, KS at 38⁰ 58’ 26’’ N, 94⁰ 43’ 54’’ W in freezing rain. The soil consisted of a mixture of clay and planting soil. I took the sample to be tested for antibiotic producing colonies against the 6 safe relatives to ESKAPE pathogens. The soil was found to have a pH of 5, and then using aseptic technique, serial dilutions were preformed and transferred to 50% TSA agar plates for growth. Plates at a 10⁻2 dilution grew the most statistically numerical colonies. A total of 12 isolated colonies were selected and grown on master plates. Each colony was then tested against the 6 safe relatives to ESKAPE pathogens on antibiotic screening plates. Two colonies produced zones of inhibition against Enterococcus faecalis, one of which was selected as the strongest candidate and a quadrant streak was performed. From the quadrant streak an isolated colony in quadrant 3 was used for Gram staining revealing a Gram-negative short bacillus. To learn more about the DNA of the bacteria, Polymerase Chain Reaction (PCR) and gel electrophoresis analysis were performed. The 16s rRNA gene will be sequenced to determine the bacteria’s genus.