Event Title

A Search for new antibiotic

Location

CoLab

Start Date

3-5-2019 9:00 AM

End Date

3-5-2019 10:15 AM

Document Type

Poster

Description

Most antibiotics that are used worldwide are derived from the soil bacteria. Therefore, the soil that we see is not just a dirt, it can be of many helps to mankind. The purpose of this study is to isolate a sample of peaty soil collected in Olathe Kansas at my backyard, to test for the presence of bacteria that could produce a new antibiotic. Serial dilution was performed, this was to help in calculating the number of cultivatable microbe present in a gram of dirt, as well as to find colonies with zone of inhibition. A Sample of each 12 colonies from original plates were transferred to a master plate using aseptic technique. They were later tested to see if they will inhibit any of the tester strains. One colony inhibited Enterobacter aerogenes, another one inhibited Enterococcus faecalis, and two others inhibited Bacillus megaterium. The streak plate of the potential candidate that inhibited E. aerogenes was done to find individual colony that will give us the bacteria DNA, which later will be amplified using polymerase chain reaction (PCR) to give us multiple copies of sequence of DNA.

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May 3rd, 9:00 AM May 3rd, 10:15 AM

A Search for new antibiotic

CoLab

Most antibiotics that are used worldwide are derived from the soil bacteria. Therefore, the soil that we see is not just a dirt, it can be of many helps to mankind. The purpose of this study is to isolate a sample of peaty soil collected in Olathe Kansas at my backyard, to test for the presence of bacteria that could produce a new antibiotic. Serial dilution was performed, this was to help in calculating the number of cultivatable microbe present in a gram of dirt, as well as to find colonies with zone of inhibition. A Sample of each 12 colonies from original plates were transferred to a master plate using aseptic technique. They were later tested to see if they will inhibit any of the tester strains. One colony inhibited Enterobacter aerogenes, another one inhibited Enterococcus faecalis, and two others inhibited Bacillus megaterium. The streak plate of the potential candidate that inhibited E. aerogenes was done to find individual colony that will give us the bacteria DNA, which later will be amplified using polymerase chain reaction (PCR) to give us multiple copies of sequence of DNA.