Candidate #5
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Location
CoLab
Start Date
3-5-2019 9:00 AM
End Date
3-5-2019 10:15 AM
Document Type
Poster
Description
Screening soil sample for potential candidate for antibiotic against ESKAPE pathogens is the focus of this project. I originally set out to test a sample of soil from my garden in Olathe which contained a combination of store-bought garden soil, and compost soil and mulch from Olathe compost site. I thought that a soil with a variety of organic matter from a variety of places would be bound to produce potential antibiotic candidates, but there was none. So, using soil sample provided in the microbiology lab at the Johnson County Community College, I carried out five 10-fold dilutions of a 1.00 g sample of the soil and plated 1mL each of 10-1, 10-2, 10-3, 10-4 and 10-5 on solid agar media. Out of seven potential candidates from the 10-2 dilution, candidate #5 when tested against tester strains, had a zone of inhibition against Staphylococcus epidermidis and Escherichia coli. A streak plate was made of candidate #5 to isolate a pure culture. The pure culture will be used for identification using genetic testing or PCR 16S gene. DNA will be sequenced and evaluated to see if candidate #5 might potentially be used for a new antibiotic against ESCAPE pathogens. In addition, we will be characterizing the bacteria using staining and metabolic testing.
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Candidate #5
CoLab
Screening soil sample for potential candidate for antibiotic against ESKAPE pathogens is the focus of this project. I originally set out to test a sample of soil from my garden in Olathe which contained a combination of store-bought garden soil, and compost soil and mulch from Olathe compost site. I thought that a soil with a variety of organic matter from a variety of places would be bound to produce potential antibiotic candidates, but there was none. So, using soil sample provided in the microbiology lab at the Johnson County Community College, I carried out five 10-fold dilutions of a 1.00 g sample of the soil and plated 1mL each of 10-1, 10-2, 10-3, 10-4 and 10-5 on solid agar media. Out of seven potential candidates from the 10-2 dilution, candidate #5 when tested against tester strains, had a zone of inhibition against Staphylococcus epidermidis and Escherichia coli. A streak plate was made of candidate #5 to isolate a pure culture. The pure culture will be used for identification using genetic testing or PCR 16S gene. DNA will be sequenced and evaluated to see if candidate #5 might potentially be used for a new antibiotic against ESCAPE pathogens. In addition, we will be characterizing the bacteria using staining and metabolic testing.
Comments
The faculty supervisor for this project was Heather Seitz, Biology.