Antibiotics of my garden
Location
CoLab, OCB 100
Start Date
27-4-2018 1:30 PM
Document Type
Poster
Description
Did you know that most antibiotics come from soil and fungi? In 1928, Alexander Fleming accidently discovered penicillin when he failed to clean out his lab before going on a vacation, but it wasn’t till the 1940 that antibiotics were really used! In class, we collected samples from our homes to test to see if we have any antibiotics growing in our backyards. After we collected our soil, we used serial dilution to grow colonies on plates. After growing colonies, we picked out the colonies that made zones of inhibition. Isolated those colonies on plates and then we tested them against gram positive and gram-negative bacteria to see if there was any effect. Fortunately, for me I have three potential colonies to test further. Biochemical test and sequencing will confirm the identity of these antibiotic producing candidates.
Image
Antibiotics of my garden
CoLab, OCB 100
Did you know that most antibiotics come from soil and fungi? In 1928, Alexander Fleming accidently discovered penicillin when he failed to clean out his lab before going on a vacation, but it wasn’t till the 1940 that antibiotics were really used! In class, we collected samples from our homes to test to see if we have any antibiotics growing in our backyards. After we collected our soil, we used serial dilution to grow colonies on plates. After growing colonies, we picked out the colonies that made zones of inhibition. Isolated those colonies on plates and then we tested them against gram positive and gram-negative bacteria to see if there was any effect. Fortunately, for me I have three potential colonies to test further. Biochemical test and sequencing will confirm the identity of these antibiotic producing candidates.
Comments
The faculty supervisor for this project was Melissa Beaty, Biology.