Event Title

Towards the Isolation and Identification of Antimicrobial Compounds Produced by Lysinibacillus pakistanensis and the Initial Screening of Compounds Isolated from Bacillus pumilus.

Location

CoLab, OCB 100

Start Date

28-4-2017 11:00 AM

End Date

28-4-2017 12:45 PM

Document Type

Poster

Description

Lysinibacillus pakistanensis was previously shown to inhibit the growth of E. faecalis, E. coli, A. faecalis, and P. aeruginosa when grown competitively using a cross streak method. Organic extracts from Lysinibacillus pakistanensis showed inhibition towards S. epidermidis and E. aerogenes in subsequent bioassays using TLC–bioautography. Further work to separate the components in the extract mixture and isolate the antimicrobial compound using both column chromatography and TLC will be discussed. Bacillus pumilus has been reported to produce unidentified antimicrobial compounds. Additionally, Bacillus pumilus was previously shown to inhibit the growth of S. epidermidis when grown competitively using a cross streak method. Results from TLC-bioautography of the organic extract from Bacillus pumilus will also be discussed.

Comments

The faculty supervisor on this project is Melanie Harvey, Chemistry.

This document is currently not available here.

Share

COinS
 
Apr 28th, 11:00 AM Apr 28th, 12:45 PM

Towards the Isolation and Identification of Antimicrobial Compounds Produced by Lysinibacillus pakistanensis and the Initial Screening of Compounds Isolated from Bacillus pumilus.

CoLab, OCB 100

Lysinibacillus pakistanensis was previously shown to inhibit the growth of E. faecalis, E. coli, A. faecalis, and P. aeruginosa when grown competitively using a cross streak method. Organic extracts from Lysinibacillus pakistanensis showed inhibition towards S. epidermidis and E. aerogenes in subsequent bioassays using TLC–bioautography. Further work to separate the components in the extract mixture and isolate the antimicrobial compound using both column chromatography and TLC will be discussed. Bacillus pumilus has been reported to produce unidentified antimicrobial compounds. Additionally, Bacillus pumilus was previously shown to inhibit the growth of S. epidermidis when grown competitively using a cross streak method. Results from TLC-bioautography of the organic extract from Bacillus pumilus will also be discussed.