Event Title

Towards the Isolation and Identification of Antimicrobial Compounds Produced by Bacillus safensis and the Initial Screening of Compounds Isolated from Lsinibacillus xylanilyticus.

Location

CoLab, OCB 100

Start Date

28-4-2017 11:00 AM

End Date

28-4-2017 12:45 PM

Document Type

Poster

Description

Bacillus safensis was previously shown to inhibit the growth of E. coli when grown competitively using a cross streak method. Organic extracts from Bacillus safensis showed inhibition towards E. coli and partial inhibition towards P. putida in subsequent bioassays using TLC–bioautography. Further work to separate the components in the extract mixture and isolate the antimicrobial compound using both column chromatography and TLC will be discussed. Lsinibacillus xylanilyticus was previously shown to inhibit the growth of E. coli, E. aerogenes, A. faecalis, P. aeruginosa, and Mycobacterium smegmatis when grown competitively using a cross streak method. Results from TLC-bioautography of the organic extract from Lsinibacillus xylanilyticus will also be discussed.

Comments

The faculty supervisor on this project is Melanie Harvey, Chemistry.

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Apr 28th, 11:00 AM Apr 28th, 12:45 PM

Towards the Isolation and Identification of Antimicrobial Compounds Produced by Bacillus safensis and the Initial Screening of Compounds Isolated from Lsinibacillus xylanilyticus.

CoLab, OCB 100

Bacillus safensis was previously shown to inhibit the growth of E. coli when grown competitively using a cross streak method. Organic extracts from Bacillus safensis showed inhibition towards E. coli and partial inhibition towards P. putida in subsequent bioassays using TLC–bioautography. Further work to separate the components in the extract mixture and isolate the antimicrobial compound using both column chromatography and TLC will be discussed. Lsinibacillus xylanilyticus was previously shown to inhibit the growth of E. coli, E. aerogenes, A. faecalis, P. aeruginosa, and Mycobacterium smegmatis when grown competitively using a cross streak method. Results from TLC-bioautography of the organic extract from Lsinibacillus xylanilyticus will also be discussed.