Towards the Isolation and Identification of Antimicrobial Compounds Produced by Bacillus safensis and the Initial Screening of Compounds Isolated from Lsinibacillus xylanilyticus.
Location
CoLab, OCB 100
Start Date
28-4-2017 11:00 AM
End Date
28-4-2017 12:45 PM
Document Type
Poster
Description
Bacillus safensis was previously shown to inhibit the growth of E. coli when grown competitively using a cross streak method. Organic extracts from Bacillus safensis showed inhibition towards E. coli and partial inhibition towards P. putida in subsequent bioassays using TLC–bioautography. Further work to separate the components in the extract mixture and isolate the antimicrobial compound using both column chromatography and TLC will be discussed. Lsinibacillus xylanilyticus was previously shown to inhibit the growth of E. coli, E. aerogenes, A. faecalis, P. aeruginosa, and Mycobacterium smegmatis when grown competitively using a cross streak method. Results from TLC-bioautography of the organic extract from Lsinibacillus xylanilyticus will also be discussed.
Image
Towards the Isolation and Identification of Antimicrobial Compounds Produced by Bacillus safensis and the Initial Screening of Compounds Isolated from Lsinibacillus xylanilyticus.
CoLab, OCB 100
Bacillus safensis was previously shown to inhibit the growth of E. coli when grown competitively using a cross streak method. Organic extracts from Bacillus safensis showed inhibition towards E. coli and partial inhibition towards P. putida in subsequent bioassays using TLC–bioautography. Further work to separate the components in the extract mixture and isolate the antimicrobial compound using both column chromatography and TLC will be discussed. Lsinibacillus xylanilyticus was previously shown to inhibit the growth of E. coli, E. aerogenes, A. faecalis, P. aeruginosa, and Mycobacterium smegmatis when grown competitively using a cross streak method. Results from TLC-bioautography of the organic extract from Lsinibacillus xylanilyticus will also be discussed.
Comments
The faculty supervisor on this project is Melanie Harvey, Chemistry.