Antibiotic Resistance With Soil

Location

CoLab, OCB 100

Start Date

28-4-2017 11:00 AM

End Date

28-4-2017 12:45 PM

Document Type

Poster

Description

Nobody can entirely avoid the threat of antibiotic resistance. Microbes will always figure out a way to fight the effects of antibiotics developed by humans. If the body started to resist medication the body needed to survive, the individual could die from an infection or have extensive recovery time. The reason behind this entire experiment is to search for new bacteria that are making antibiotics. To find bacteria, we had to find the soil we were going to be plating with. My soil is from farm land (not with crops or anything else to contaminate the soil) and has latitude of 39.17 and longitude of -94.93. To begin the experiment, we plated soil samples onto agar media, watched them grow, and determined zones of inhibition. After we determined the zones of inhibition, we then put those bacteria on a patch plate and let them grow. After our patch plates grew up, we did proof plating to discover if any of our potential candidates had the possibility to combat any of the ESKAPE pathogens. If we found that a certain bacteria could combat one or many of the ESKAPE pathogens, we went back to our patch plate and took the same bacteria to make a streak plate. So far I have found that some of my bacteria is resistant to some pathogens.

Comments

The faculty supervisor on this project is Heather Seitz, Biology.

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Apr 28th, 11:00 AM Apr 28th, 12:45 PM

Antibiotic Resistance With Soil

CoLab, OCB 100

Nobody can entirely avoid the threat of antibiotic resistance. Microbes will always figure out a way to fight the effects of antibiotics developed by humans. If the body started to resist medication the body needed to survive, the individual could die from an infection or have extensive recovery time. The reason behind this entire experiment is to search for new bacteria that are making antibiotics. To find bacteria, we had to find the soil we were going to be plating with. My soil is from farm land (not with crops or anything else to contaminate the soil) and has latitude of 39.17 and longitude of -94.93. To begin the experiment, we plated soil samples onto agar media, watched them grow, and determined zones of inhibition. After we determined the zones of inhibition, we then put those bacteria on a patch plate and let them grow. After our patch plates grew up, we did proof plating to discover if any of our potential candidates had the possibility to combat any of the ESKAPE pathogens. If we found that a certain bacteria could combat one or many of the ESKAPE pathogens, we went back to our patch plate and took the same bacteria to make a streak plate. So far I have found that some of my bacteria is resistant to some pathogens.