Event Title

Newly discovered bacteria, SA27C, possible inhibitor of the deadly pathogen Escherichia coli

Location

CoLab, OCB 100

Start Date

28-4-2017 1:00 PM

End Date

28-4-2017 2:45 PM

Document Type

Poster

Description

In the pharmaceutical businesses, new antibiotics against various pathogenic agents are being made everyday. However, there are many pathogens that grow resistant to antibiotics rapidly, thus new antibiotics still need to be discovered that work against these pathogens. Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterobacter species, are some of these pathogens that directly affect humans. Many antibiotics are discovered from soil of different geographical locations. The aim of the current study in the lab was to collect soil and perform an experiment to check if the microorganisms within the soil can inhibit the growth of the pathogens Enterococcus faecium, Staphylococcus aureus, Escherichia coli, Pseudomonas putida, Enterobacter aerogenes. These pathogens are safe relatives of the previously described pathogens. The soil in this experiment was collected from 38° 55’10’’ N 94°35’13’’ W, Kansas City, MO. Then the soil was diluted using the 10-1, 10-2,10-4, 10-5, 10-6 dilution factors. Each diluted soil sample was transferred to 50% TSA agar media and autoclaved. Possible candidates were observed and then plated on a patch plate. The cavalier method was used to check if the possible candidates would inhibit any of the relative pathogens. Candidate C from the 10-2 dilution sample showed a possible zone of inhibition around E. coli. A streak plate was made from candidate C. The isolated form of the possible candidate will further be studied on a microscopic level. This research will lead to better understand the pathogen E. coli and what qualities should microbes have to resist E. coli.

Comments

The faculty supervisor on this project is Heather Seitz, Biology.

This document is currently not available here.

Share

COinS
 
Apr 28th, 1:00 PM Apr 28th, 2:45 PM

Newly discovered bacteria, SA27C, possible inhibitor of the deadly pathogen Escherichia coli

CoLab, OCB 100

In the pharmaceutical businesses, new antibiotics against various pathogenic agents are being made everyday. However, there are many pathogens that grow resistant to antibiotics rapidly, thus new antibiotics still need to be discovered that work against these pathogens. Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterobacter species, are some of these pathogens that directly affect humans. Many antibiotics are discovered from soil of different geographical locations. The aim of the current study in the lab was to collect soil and perform an experiment to check if the microorganisms within the soil can inhibit the growth of the pathogens Enterococcus faecium, Staphylococcus aureus, Escherichia coli, Pseudomonas putida, Enterobacter aerogenes. These pathogens are safe relatives of the previously described pathogens. The soil in this experiment was collected from 38° 55’10’’ N 94°35’13’’ W, Kansas City, MO. Then the soil was diluted using the 10-1, 10-2,10-4, 10-5, 10-6 dilution factors. Each diluted soil sample was transferred to 50% TSA agar media and autoclaved. Possible candidates were observed and then plated on a patch plate. The cavalier method was used to check if the possible candidates would inhibit any of the relative pathogens. Candidate C from the 10-2 dilution sample showed a possible zone of inhibition around E. coli. A streak plate was made from candidate C. The isolated form of the possible candidate will further be studied on a microscopic level. This research will lead to better understand the pathogen E. coli and what qualities should microbes have to resist E. coli.