Event Title

New Bacteria to Fight Antibiotic Resistance

Location

CoLab, OCB 100

Start Date

28-4-2017 1:00 PM

End Date

28-4-2017 2:45 PM

Document Type

Poster

Description

As a result of continual over use of antibiotics, bacterial strains have become increasingly able to withstand the effects of antibiotics. These bacteria, specifically the ESKAPE pathogens, should they persist without any new antibiotics to fight them, will continue to be and become more of a life-threatening issue for anyone exposed either in a hospital, traveling abroad or just scrapping their knee at the playground. To solve this problem, I’ve screened many bacteria using soil from places that seem to be potentially good places for a diverse group of healthy bacteria to grow. I found my soil sample by a healthy tree at Broken Arrow park, 2800 Louisiana St. Lawrence, Kansas on January 30, 2017. The weather when the soil sample at the time of soil collection was sunny and 62 degrees, relatively warm, and the soil itself was slightly damp and clumpy. After dilutions and creating a plate of distinct colonies close enough together to express zones of inhibition were made those that created a zone were collected. When tested against the ESKAPE pathogen relatives, I’ve found one candidate that inhibited the growth of Staphylococcus epidermis. This candidate is dry, flat, white with round, uniform edges. This candidate was then used to make a streak plate to properly isolate colonies of it and will be further tested to find its specific DNA sequence in regards to the 16s rRNA gene.

Comments

The faculty supervisor on this project is Heather Seitz, Biology.

Streaming Media

Image

This document is currently not available here.

Share

COinS
 
Apr 28th, 1:00 PM Apr 28th, 2:45 PM

New Bacteria to Fight Antibiotic Resistance

CoLab, OCB 100

As a result of continual over use of antibiotics, bacterial strains have become increasingly able to withstand the effects of antibiotics. These bacteria, specifically the ESKAPE pathogens, should they persist without any new antibiotics to fight them, will continue to be and become more of a life-threatening issue for anyone exposed either in a hospital, traveling abroad or just scrapping their knee at the playground. To solve this problem, I’ve screened many bacteria using soil from places that seem to be potentially good places for a diverse group of healthy bacteria to grow. I found my soil sample by a healthy tree at Broken Arrow park, 2800 Louisiana St. Lawrence, Kansas on January 30, 2017. The weather when the soil sample at the time of soil collection was sunny and 62 degrees, relatively warm, and the soil itself was slightly damp and clumpy. After dilutions and creating a plate of distinct colonies close enough together to express zones of inhibition were made those that created a zone were collected. When tested against the ESKAPE pathogen relatives, I’ve found one candidate that inhibited the growth of Staphylococcus epidermis. This candidate is dry, flat, white with round, uniform edges. This candidate was then used to make a streak plate to properly isolate colonies of it and will be further tested to find its specific DNA sequence in regards to the 16s rRNA gene.